Quality of life is affected by people’s cultures, situations, expectations, goals, standards, and value systems. This study aims to determine the quality of life of students in terms of their relations with academicians, other students and administrative personnel, opportunities offered by the university, and their satisfaction level with their university in three major Turkish universities in 2016-2017 academic school year. A total of 417 participants including 131 males and 286 females were selected through a non-random selection. The study included a mixed method approach. The survey model of descriptive method was used in the quantitative part of the study. The qualitative part of the study included a semi-structured interview technique involving open ended questions. The quantitative data was analyzed according to the research questions using one-way analysis of variances (ANOVA), Pearson’s correlation, and stepwise regression analysis. In addition, content analysis method was employed to analyze qualitative data. The findings showed that university students were uncertain about factors affecting their quality of life.